Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > PCR - Polymerase Chain Reaction Forum
Register Search Today's Posts Mark Forums Read

PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


cDNA left in thermocycler at 94C for ~20 min.

cDNA left in thermocycler at 94C for ~20 min. - PCR - Polymerase Chain Reaction Forum

cDNA left in thermocycler at 94C for ~20 min. - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 02-13-2012, 12:51 AM
Pipette Filler
Points: 120, Level: 2 Points: 120, Level: 2 Points: 120, Level: 2
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2012
Posts: 3
Thanks: 1
Thanked 0 Times in 0 Posts
Default cDNA left in thermocycler at 94C for ~20 min.



Before running a PCR, I paused my cycling program to let it heat up. I then added my cDNA samples and THOUGHT that had I unpaused it to begin the cycling...
However, I went to check on the samples roughly 20 min. later and noticed that the program was still paused at 94C and the cycling never began...I went ahead and started it anyway...

How will this affect my PCR products?..
Reply With Quote
  #2  
Old 02-15-2012, 04:22 AM
admin's Avatar
Administrator
 
Join Date: Nov 2005
Posts: 1,418
Thanks: 883
Thanked 68 Times in 58 Posts
Default Re: cDNA left in thermocycler at 94C for ~20 min.

Hi there
No at 94 C no enzymes will degrade your DNA or RNA.

The only issue will be if your cDNA was double stranded it would have become single stranded.

Is your cDNA ss or ds?

cheers
Reply With Quote
Reply

Tags
94c , cdna , left , min , thermocycler


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Help needed on aminoallyl-labeled cDNA palver Molecular Biology Articles and Protocols 0 01-07-2009 08:40 AM
cDNA Synthesis and Amplification of POLY(A) mRNA by RT-PCR Protocol domba Real-Time PCR and Quantitative PCR Forum 3 09-09-2008 02:48 PM
Commercial cDNA or cdna Synthesized Random hemer for Transfection bhunni DNA Techniques 0 09-01-2007 07:12 AM
cDNA precipitation without coprecipitation of primers Simone Marker Protocols and Methods Forum 1 03-05-2007 08:36 PM


All times are GMT. The time now is 05:42 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11495 seconds with 16 queries