I have a problem of no amplification with my primers. The problem is: the PCR give amplification because the primers are well designed but after a while this PCR does not work. The first time that it didn't give amplification I changed all the components included the ADN and still did not work. After I buy new primers the first two amplifications were good and after and then still did not work. My laboratory members also have the same problem.
May be what are the primers unstable or fault of dilute?
The solvent where we dilute the primers is TE ( Tris 0,01 M, EDTA-Na2 0,004 Mm, pH 8).