[Only registered users see links. ] Enter one or both primer sequences in the Primer Parameters section of the form. If only one primer is available, a template sequence is also required. See "A Target Template Sequence.
In the Primer Pair Specificity Checking Parameters section, select the appropriate source Organism and the smallest Database that is likely to contain the target sequence. These settings give the most precise results. For broadest coverage, choose the nr database and do not specify an organism.
Click the "Get Primers" button to submit the search and retrieve template and specificity information.