I have a question about making solutions etc
(Stock solution) I have a 794 pmol/ul of my forward primer, which is x100 and I need to make a x10 working solution which i will use for the PCR reaction. I need to have 2.5 ul of the forward primer for the reaction.
So how will I make a x10 working solution from a x100 stock solution ?
and also, what would be the concentration of this primer? how can you calculate concentration of it ?
Please someone help as I am stuck and this is really important for me.