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Why are DNA primers used in PCR but RNA primers used in DNA replication?

Why are DNA primers used in PCR but RNA primers used in DNA replication? - PCR - Polymerase Chain Reaction Forum

Why are DNA primers used in PCR but RNA primers used in DNA replication? - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 12-03-2011, 01:17 AM
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Default Why are DNA primers used in PCR but RNA primers used in DNA replication?



Just a little confused. Thanks in advance.
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Old 12-03-2011, 02:17 PM
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Default Re: Why are DNA primers used in PCR but RNA primers used in DNA replication?

I believe your question is partially incomplete or wrong. Neither process requires any RNA primers.

What do you mean by DNA replication? If it's a natural biological process in the cell, it doesn't involve RNA primers.

If it's a synthetic process in the lab, then DNA replication would likely be in the form of amplifying specific regions of DNA. And well, DNA primers are more stable than RNA in vitro and easier/cheaper to synthesise.
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dna , pcr , primers , replication , rna


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