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Why are DNA primers used in PCR but RNA primers used in DNA replication?

Why are DNA primers used in PCR but RNA primers used in DNA replication? - PCR - Polymerase Chain Reaction Forum

Why are DNA primers used in PCR but RNA primers used in DNA replication? - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 12-02-2011, 06:17 PM
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Default Why are DNA primers used in PCR but RNA primers used in DNA replication?



Just a little confused. Thanks in advance
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Old 12-02-2011, 06:17 PM
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In PCR, you are trying to isolate a section of the DNA to replicate. Therefore, you would use DNA primers to attach to the section of DNA of interest. Most of the primers have some form of a tag on them to be found.

DNA cannot be replicated in lab or in nature without a primer to use as a starting point. This is usually a short strand of RNA (both in lab and in nature).
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Old 12-02-2011, 07:17 PM
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Default Re: Why are DNA primers used in PCR but RNA primers used in DNA replication?

Quote:
Originally Posted by Foxy View Post
In PCR, you are trying to isolate a section of the DNA to replicate. Therefore, you would use DNA primers to attach to the section of DNA of interest. Most of the primers have some form of a tag on them to be found.

DNA cannot be replicated in lab or in nature without a primer to use as a starting point. This is usually a short strand of RNA (both in lab and in nature).
LOL, what are you smoking? I'm gonna go with DNA primers are stable, why would you want to use RNA primers doing a PCR?
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dna , pcr , primers , replication , rna


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