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-   -   Why are DNA primers used in PCR but RNA primers used in DNA replication? (http://www.molecularstation.com/forum/pcr-polymerase-chain-reaction-forum/84212-why-dna-primers-used-pcr-but-rna-primers-used-dna-replication.html)

all.smiles 12-02-2011 06:17 PM

Why are DNA primers used in PCR but RNA primers used in DNA replication?
 
Just a little confused. Thanks in advance

Foxy 12-02-2011 06:17 PM

In PCR, you are trying to isolate a section of the DNA to replicate. Therefore, you would use DNA primers to attach to the section of DNA of interest. Most of the primers have some form of a tag on them to be found.

DNA cannot be replicated in lab or in nature without a primer to use as a starting point. This is usually a short strand of RNA (both in lab and in nature).

pigfarmer 12-02-2011 07:17 PM

Re: Why are DNA primers used in PCR but RNA primers used in DNA replication?
 
Quote:

Originally Posted by Foxy (Post 437490)
In PCR, you are trying to isolate a section of the DNA to replicate. Therefore, you would use DNA primers to attach to the section of DNA of interest. Most of the primers have some form of a tag on them to be found.

DNA cannot be replicated in lab or in nature without a primer to use as a starting point. This is usually a short strand of RNA (both in lab and in nature).

LOL, what are you smoking? I'm gonna go with DNA primers are stable, why would you want to use RNA primers doing a PCR?


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