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Help needed with this PCR reaction problem?

Help needed with this PCR reaction problem? - PCR - Polymerase Chain Reaction Forum

Help needed with this PCR reaction problem? - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 11-05-2011, 08:17 PM
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Default Help needed with this PCR reaction problem?



Does anyone have an ideas for this problem:

1 microgram of human genomic DNA is amplified in a PCR reaction using primers designed to amplify a 330 base pair fragment.

After 30 cycles, calculate how much PCR product (in micrograms) could potentially be produced.

Useful information
Length of human genome = 3x10^9 base pairs
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Old 11-06-2011, 04:51 AM
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Default Re: Help needed with this PCR reaction problem?

that would definitely depend on
a) unit of taq polymerase.
b) efficiency of the PCR.
c) other etc etc...
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