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2. Using PCR and gel electrophoresis (use both), how will you determine

2. Using PCR and gel electrophoresis (use both), how will you determine - PCR - Polymerase Chain Reaction Forum

2. Using PCR and gel electrophoresis (use both), how will you determine - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 10-23-2011, 04:16 AM
Pipette Filler
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Default 2. Using PCR and gel electrophoresis (use both), how will you determine



which tube carries which sample? 2. Let's say you have two bacterial samples in tubes 1 and 2, one of which has been transformed with ONLY a vector and the other with a RECOMBINANT construct. Using PCR and gel electrophoresis (use both), how will you determine which tube carries which sample? Describe where the primers will anneal (not specific sequence but general location), what you expect to observe on the gel and how you will analyze and draw conclusions from the results? A schematic may be helpful!
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