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When running a PCR (polymerase chain reaction) heating up the reaction causes?

When running a PCR (polymerase chain reaction) heating up the reaction causes? - PCR - Polymerase Chain Reaction Forum

When running a PCR (polymerase chain reaction) heating up the reaction causes? - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 10-08-2011, 07:16 PM
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Default When running a PCR (polymerase chain reaction) heating up the reaction causes?



a. the hydrogen bonds in DNA to weaken so the strands separate
b. the polymerase to read the DNA strand faster
c. nucleotides to separate from the original strand
d. restriction enzymes to be formed
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Old 10-08-2011, 07:17 PM
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A. Heating weakens the hydrogen bonds holding both strands together, eventually allowing them to be separated and replicated.
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Old 10-08-2011, 07:17 PM
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answers is a.

When DNA is heated, denaturation of DNA takes place. Where the hydrogen bonds break leading to separation of strands.
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