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| PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory. |
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#1
10-07-2011, 08:06 PM
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 Papd pcr Hi every one please, I need to get a practical information about RAPD PCR, such as standard protocols, tricks, ......, and how to read the results of PCR product by gel electrophoresis. I read many articles about this subject, now I want to start my project on genetic disease by using this technique (RAPD PCR) so, please I need your advices in this matter. waiting your answer  Last edited by Hano; 10-07-2011 at 08:07 PM. Reason: RAPD  |
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#2
01-23-2013, 06:18 AM
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| Re: Papd pcr [Only registered users see links. ]The scientist will design a primer with an arbitrary sequence. In other words, the scientist simply makes up a 10 base pair sequence (or may have a computer randomly generate a 10 bp sequence), then synthesizes the primer. The scientist then carries out a PCR reaction and runs an agarose gel to see if any DNA segments were amplified in the presence of the arbitrary primer. |
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