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PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


Papd pcr

Papd pcr - PCR - Polymerase Chain Reaction Forum

Papd pcr - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 10-07-2011, 08:06 PM
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Default Papd pcr



Hi every one

please, I need to get a practical information about RAPD PCR, such as standard protocols, tricks, ......, and how to read the results of PCR product by gel electrophoresis.
I read many articles about this subject, now I want to start my project on genetic disease by using this technique (RAPD PCR) so, please I need your advices in this matter.

waiting your answer

Last edited by Hano; 10-07-2011 at 08:07 PM. Reason: RAPD
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Old 01-23-2013, 07:18 AM
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Default Re: Papd pcr

[Only registered users see links. ]The scientist will design a primer with an arbitrary sequence. In other words, the scientist simply makes up a 10 base pair sequence (or may have a computer randomly generate a 10 bp sequence), then synthesizes the primer. The scientist then carries out a PCR reaction and runs an agarose gel to see if any DNA segments were amplified in the presence of the arbitrary primer.
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