Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum how to dilute 100mM dNTP to 1.25mM? what is the calculation to make pcr reaction...

 PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.

# how to dilute 100mM dNTP to 1.25mM? what is the calculation to make pcr reaction...

## how to dilute 100mM dNTP to 1.25mM? what is the calculation to make pcr reaction... - PCR - Polymerase Chain Reaction Forum

### how to dilute 100mM dNTP to 1.25mM? what is the calculation to make pcr reaction... - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.

#1
09-21-2011, 11:16 PM
Pipette Filler

Join Date: Sep 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
how to dilute 100mM dNTP to 1.25mM? what is the calculation to make pcr reaction...

...work solution? I have stock of 100mM dNTP. I need to make 1.25mM for 25ul pcr reaction.I am confused about the calculation.If anybody help me please...
#2
09-22-2011, 05:50 AM
Volunteer

Join Date: Oct 2010
Location: spain
Posts: 58
Thanks: 1
Thanked 27 Times in 25 Posts
Re: how to dilute 100mM dNTP to 1.25mM? what is the calculation to make pcr reaction.

hola, 100/1.25=80 so you have to dilute your solution 80 times to have 1.25mM. But if this concentration is the final in reaction mixture, 25/80=0.3125ul 0r 3.12 ul of an intermediate solution 10mM (10ul of 100mM +90 H2O). Buena suerte
#3
09-23-2011, 09:18 AM
Pipette Filler

Join Date: Jan 2010
Location: Norway
Posts: 22
Thanks: 0
Thanked 4 Times in 4 Posts
Re: how to dilute 100mM dNTP to 1.25mM? what is the calculation to make pcr reaction.

The best and the most used formula I ever learned at school is this one:
c1*v1=c2*v2 where:
c1= the concentration of the solution you want to dilute
v2= the volume you should use of the solution
c2= the concentration you want in the final solution
v2= The volume you want of the final solution

So lets say you want to make 500 ul of a 12,5 mM solution the formula is:
c1*v1=c2*v2
100 mM * v1 = 12,5 mM * 500 ul
v1 = 62,5 ul
That means you have to use 62,5 ul of your 100 mM solution and dilute it in 437,5 ul of whatever you want to dilute it in to get a final volume of 500 ul. Then you have a 12,5 mM solution of your primers, and you can then use 2,5 ul of this solution in your 25 ul to get a final concentration in the reaction of 1,25 mM.
 The Following User Says Thank You to Aurora Borealis For This Useful Post: butters (09-29-2011)
#4
12-10-2011, 05:23 AM
Pipette Filler

Join Date: Dec 2011
Posts: 16
Thanks: 0
Thanked 4 Times in 4 Posts
Re: how to dilute 100mM dNTP to 1.25mM? what is the calculation to make pcr reaction.

mahmoud manama

 Tags 100mm , 125mm , calculation , dilute , dntp , make , pcr , reaction

 Thread Tools Display Modes Linear Mode

 Posting Rules You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is OffTrackbacks are On Pingbacks are On Refbacks are On Forum Rules
 Forum Jump User Control Panel Private Messages Subscriptions Who's Online Search Forums Forums Home General Science Forums     Biology Forum     New Member Introductions Forum     Chemistry Forum         Organic Chemistry Forum     Physics Forum     General Science Questions and Layperson Board         Science and Religion Forum         Zoology Forum     Environmental Sciences and Issues General Forum     Chit Chat         Science and Lab Jokes     Article Discussion     Molecular Biology News and Announcements         Conferences , Symposiums and Meetings         Molecular Station Suggestion Forum         Instructions for Posting, Help, and Frequently Asked Questions     Science News and Views         Molecular Biology Lectures and Videos     Science Careers         Post-doctoral         Medical School         Ph.D Doctor of Philosophy         Science Jobs Forum Molecular Research Topics Forum     PCR - Polymerase Chain Reaction Forum         Real-Time PCR and Quantitative PCR Forum     Bioinformatics         BioStatistics Forum     Molecular Biology Techniques         Molecular Cloning Forum         Electrophoretic Mobility Shift Assay Forum         Agarose Gel Electrophoresis Forum         BioPhysics Forum         Gene Therapy     Cell Biology and Cell Culture         Apoptosis, Autophagy, and Necrosis Forum         Flow Cytometry Forum         Transfection Forum         Confocal - Microscopy Imaging Techniques         Immunology and Host-Pathogen Interactions         Signalling Biology         Stem Cell Forum     Basic Lab Protocols and Techniques         SDS-PAGE Gel Electrophoresis Forum     DNA Techniques         DNA Extraction Forum         cDNA Forum     Epigenetics Forum: DNA Methylation, Histone and Chromatin Study         ChIP Chromatin Immunoprecipitation Forum     Protein Science         Antibody Forum             Immunoprecipitation Forum         Western Blot Forum         Protein Crystallography Forum         Recombinant Protein Forum         ELISA Assay Forum         Protein Forum     Proteomics Forum         Peptide Forum         Mass Spectrometry Forum         2-D Gel Electrophoresis Forum     Omics and Genomics Forum         Microarrays Forum         Genomics Forum     RNA Techniques Forum         RNAi and SiRNA Forum     Histology Forum         Immunohistochemistry Forum         Immunocytochemistry Forum         Electron Microscopy Forum         Immunofluorescence Forum     Protocols and Methods Forum     Molecular Biology Articles and Protocols     Animal and Molecular Model Systems         Drosophila Forum         Yeast Forum         Zebrafish Forum         Botany Forum         C Elegans Forum         Arabidopsis and Plant Biology         Microbiology Forum         Knockout Mouse Forum     Chromatography Forum Products and Vendor Discussion     Molecular Biology Products and Vendors         Bad Product/Service? Post Here         Lab Equipment Discussion and Reviews Regional Molecular Biology Discussion     Forum Chemie     Forum Biologie     Forum Biologia     Forum Chimica     Forum Physik     Forum De Chimie     Forum De Physique     Forum Chemia     中国人分子的生物学论坛 Chinese     Greek Molecular Biology Forums     分子生物学のフォーラム Japanese     ميدان فارسى. Persian Molecular Biology     [أربيك] علم ساحة- Arabic     Forum de Biologie Moleculaire     Forum Biologia Molecolare     Forum die Molekularbiologie     Foro Biologia Molecular

 Similar Threads Thread Thread Starter Forum Replies Last Post Tania Guthrie Forum Physik 0 09-23-2009 04:43 PM jon_johnfrancisayres@yahoo.com Microbiology Forum 0 10-06-2007 05:59 AM

All times are GMT. The time now is 01:59 PM.

 Contact Us - Molecular Biology - Archive - Privacy Statement - Top