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Polymerase chain reaction?

Polymerase chain reaction? - PCR - Polymerase Chain Reaction Forum

Polymerase chain reaction? - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 09-16-2011, 04:16 AM
Pipette Filler
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Default Polymerase chain reaction?



how much magnesium chloride, primer , DNA and dNTP's in microlitres would I need for a 5 micro liter pcr mix
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Old 12-10-2011, 06:12 AM
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Default Re: Polymerase chain reaction?

1-4 mgcl2
0.5 of 0.2mM each dntb
upstream primer and down streamin final concentration :0.1-1 microlitter
DNA :less than 0.5microgram /50 microlitter
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