Polymerase chain reaction?

Dark M · #1 09-16-2011, 04:16 AM

how much magnesium chloride, primer , DNA and dNTP's in microlitres would I need for a 5 micro liter pcr mix

m37786 · #2 12-10-2011, 05:12 AM

1-4 mgcl2
0.5 of 0.2mM each dntb
upstream primer and down streamin final concentration :0.1-1 microlitter
DNA :less than 0.5microgram /50 microlitter

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