The question is will I be able to know if my strategy to separate the DNA from other cellular components (proteins, starch, etc) has succeeded for failed?
Basically, I extract the DNA from the cell using buffer and enzyme. My strategy to separte the DNA is that I add ethanol in high salt condition so that the DNA will bind to the silica gel in chromatography column. Then I wash the sample to remove the cellular stuff, centrifuge, and dilute the DNA with water. Then I do the first round of PCR.
so will I know if that strategy has worked? I'm thinking yes because if the separation failed then nothing will be amplified in PCR and we would know that right?