Originally Posted by saranyah
I didn't get the amplification.... And also no primer dimer.... The genomic DNA was checked for 16S rRNA universal primer and it showed a very good amplification... But I'm still not getting my gene out of it... I'm frustrated... Its been a month Sir...
There is no need for frustration. No protocol, No experiment works perfectly in first time,if ie the case then every one will finish there project in a week.
Once i took more than 3 month to optimize my PCR protocol.
Please write what exactly are you doing. some time you are writing bacterial DNA, then now genomic DNA. If you write each details your pcr protocol, components, Template source, expected size of your product etc etc. It will help to answer your question correctly.