I have been trying to do my first PCR but met with failures.
The 1st PCR showed 1kb bands in the agarose gel lane of my water control, pcDNA3.1 vector control, and the plasmid DNA template. I am trying to amplify DC-SIGN gene of 1200bp. I have been using plasmid DNA concn of 50ng/ul.
I did another one that showed no bands at all.
I then repeated the PCR using Supertherm Taq Polymerase instead of Phusion Taq polymerase that I used in previous reactions.
What could be wrong with my procedures?
Thanks a lot.