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#1
07-05-2011, 07:31 AM
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 Non-specific band in positive control but negative control not Dear guys, My previous PCRs were OK but recently, I have got a problem of nonspecific band in positve control. I'm sure this is not contamination in reagents because negative control is no problem. So i tend to think may be positive DNA was contaminated. But non-specific band is about 1000kb  and target band is 123bpIf my PCR process have problem, previous PCR results would have non-specific band. But they were not. PCR reaction 940C for 4 min Denaturation : 94C for 2 mins Annealing : 68C for 2 mins 35 cycles Extension : 72C for 1 mins 72C for 10 mins load DNA 5ul May be because concentration DNA was too much? It's was about 28 ng/ul   |
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| band , control , negative , nonspecific , positive |
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