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What happens to free phosphates after DNA elongation in PCR?

What happens to free phosphates after DNA elongation in PCR? - PCR - Polymerase Chain Reaction Forum

What happens to free phosphates after DNA elongation in PCR? - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 06-24-2011, 02:16 AM
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Default What happens to free phosphates after DNA elongation in PCR?



When Taq Polymerase adds free nucleotides to the 3' OH end of template DNA, 2 of the 3 phosphates of the nucleotide are removed (creating the energy necessary to form the phosphodiester bond that attaches the remaining phosphate group the the growing chain). What happens to those 2 phosphates that are removed? How does this effect the other molecules in the PCR solution?
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Old 06-24-2011, 02:20 AM
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Old 06-24-2011, 02:24 AM
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Old 06-24-2011, 02:35 AM
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