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Purpose of Boric Acid in Polymerase Chain Reaction? (On a molecular level, please)?

Purpose of Boric Acid in Polymerase Chain Reaction? (On a molecular level, please)? - PCR - Polymerase Chain Reaction Forum

Purpose of Boric Acid in Polymerase Chain Reaction? (On a molecular level, please)? - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 06-13-2011, 06:16 PM
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Default Purpose of Boric Acid in Polymerase Chain Reaction? (On a molecular level, please)?



5X TBE Buffer includes Tris Base, 0.5 M EDTA, and Boric Acid. What is the purpose of Boric Acid (in as much detail as possible, please!).
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Old 07-09-2011, 01:15 AM
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Default Re: Purpose of Boric Acid in Polymerase Chain Reaction? (On a molecular level, please

I believe that boric acid is used to keep the DNA deprotonated and soluble in water.
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