One problem in our lab is knowing whether we have had successful PCR amplification or not. We run a lot of PCR and our normal test is to run a gel afterwards to check that there's a band. This is time consuming especially when running lots of samples.
I was wondering if you know of any tips so DNA quantity can be measured *after* the PCR reaction to determine whether amplification occurred. It wouldn't have to be extremely accurate, but just give us the ability to tell the difference between "no amplification" and "amplification" without running a gel.
1. Nanodrop (don't have one, chime in if you have tried this)
2. EtBr UV spot test (haven't tried this, but have seen a few references to it)?
3. EtBr into tube, then put the tube in the imager and see if it glows, try to quantify this?
4. Other ideas?