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The name of the liquid that contains the DNA that is then transferred into the...

The name of the liquid that contains the DNA that is then transferred into the... - PCR - Polymerase Chain Reaction Forum

The name of the liquid that contains the DNA that is then transferred into the... - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 06-02-2011, 12:18 AM
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Default The name of the liquid that contains the DNA that is then transferred into the...



...PCR tube is called the supernat? True or false
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Old 06-02-2011, 03:55 PM
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Default Re: The name of the liquid that contains the DNA that is then transferred into the...

I think is false. The supernatant is the part that contains the DNA after a centrifugation, being the lower part the organic phase (proteins, lipids and stuff) and the upper part the supernatant.

Usually, you already have you DNA purified and dissolved in water or some buffer before adding it to the PCR tube. Such liquid can't be called supernatant because it is not, considering what I mention in the above paragraph.
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