smear in long distance PCR

[butters]

Maybe you could try using photobucket and paste the link here

[gautama]

Hi, sorry to bother you all.
I need some help with PCR and DGGE.

I am looking at the soil microbiota as a whole rather than isolating specific bacterial species. In my DGGE profile the intensities of each band is about the same at each lane
(seen at the same row), that's what make my head aches. I was hoping to see a different profile lane to lane, but it seems they were all look the same! But from one band to another in the same lane/column have different intensities. I used 338F-GC and 518R. Any ideas how can I avoid two bacterial species from stopping at the same point in my DGGE gel beside using different primers?
Thanks for your kind answer...

[gautama]

Hi, sorry to bother you all, but maybe you can lend me a hand for my problem

I am looking at the soil microbiota as a whole rather than isolating specific bacterial species. In my DGGE profile the intensities of each band is about the same at each lane
(seen at the same row), that's what make my head aches. I was hoping to see a different profile lane to lane, but it seems they were all look the same! But from one band to another in the same lane/column have different intensities. I used 338F-GC and 518R. Any ideas how can I avoid two bacterial species from stopping at the same point in my DGGE gel beside using different primers?
Thanks for your kind answer...

[butters]

Gautama, it would be great if you make a new topic on it. I may have some idea but would need some detail again.

Lina, is everything ok with your result? Generally I would prefer ppl to post here so everybody can have a look and maybe uncover something we overlook. But if you insist I could ahem give out my email. and with your permission to post those picture you of course. If require.

[Lina Sun]

Sorry, I am a freshman in PCR, and I didn't carry out DGGE.