|Register||Search||Today's Posts||Mark Forums Read|
|PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.|
| ||LinkBack||Thread Tools||Display Modes|
PCR wrong size bands?
Hey guys, I'm doing chIP. A lab mate of mine had the particular primers I'm using made about 1.5 years ago. I used them 1 year ago and got the correct size product of 250bp. I'm repeating the same chip with a different antibody and I'm getting a 150bp product in the IP'd and input samples every single time. I've varied the polymerase from PFU to Taq and I've changed temperatures. My question is, could the primers be degraded. Has anyone experienced this phenomenon of constantly getting the same wrong sized band due to that? The sizes for my controls and the primers I created are all correct.
Re: PCR wrong size bands?
the primers probably are not the problem. Try making new primer batch from the stock although I don't think that would help.
Maybe you are using a different cycling program for PCR than him? Are you using different buffers or salts than him?
Also maybe your DNA is degraded?
Just some ideas! cheers
|bands , pcr , size , wrong|
|Thread||Thread Starter||Forum||Replies||Last Post|
|PCR negative control contamination with bands of a different size!!||Luli||PCR - Polymerase Chain Reaction Forum||4||07-01-2010 07:46 PM|
|Bands in wrong place!||halfro22||Western Blot Forum||0||03-09-2010 10:12 PM|
|bands always in wrong spot!||jess12||Western Blot Forum||5||12-20-2009 03:54 PM|
|Bands in the wrong place||jasmith456||Western Blot Forum||2||06-08-2009 01:35 PM|
|GNU units and units.dat; Units of Measurement and Unit Conversion||James Redford||Physics Forum||0||07-31-2005 12:08 PM|