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Primer contamination

Primer contamination - PCR - Polymerase Chain Reaction Forum

Primer contamination - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 04-11-2011, 06:47 AM
Pipette Filler
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Default Primer contamination



Dear everyone
I research on GLUT4 gene expression but I suspected that my primer is contaminated with DNA because in NTC, I observed the same band of GLUT4 in PCR gel electrophoresis.
Please help me and introduce me the paper that show the methods that give confidence the primer is contaminated or not.
Thanks
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Old 03-28-2013, 06:30 AM
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Default Re: Primer contamination

Try using a fresh aliquot of primer stock. contamination can not only from primers, there are various things you should look into.
your reagents, handling practices, pipettes, lab cleaning practices, etc.
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