I am new to this but will be really grateful if someone knowledgeable can help me with this. I am trying to design primers for RT-PCR. I am assuming here that I will have to find an mRNA sequence of interest and then design complementary primers for the mRNA sequence.
When I search for mRNA sequence in nucleotide or genebank I get sequences that do not have Uracil (u) but have thymine (T) in the sequence. Should the RNA not have (u) substituted for (t)?
'f9or eg search for " Mus musculus platelet derived growth factor receptor, beta polypeptide (Pdgfrb), transcript variant 1, mRNA" in nucleotides. Is the sequence displayes of 1) mRNA, 2) cDNA or 3) orginal gene (DNA).