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| PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory. |
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#1
03-20-2011, 01:25 AM
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 qRT-PCR Hello all. I started my RT-PCR but I was confused with somthing.. I do have 2 house keeping gene, two tissue and 4 genes (8 primers) I want to do 4 dilution and undiluted sample for each gene which sample should I assigned as standered and unkown to have a standered curve and what is the intial concentration for each sample diluted and undiluted as I was asked by the program I need help please as soon as possible do you know any helpful website as well thank and all  |
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#2
03-20-2011, 01:43 AM
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| Re: qRT-PCR When I set the samples in the machine it say standard sample should have concentration above zero and I get a box with the following equation 1.E+06 what is this equation and how i use it to proced with next step |
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