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PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


qRT-PCR

qRT-PCR - PCR - Polymerase Chain Reaction Forum

qRT-PCR - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 03-20-2011, 01:25 AM
Pipette Filler
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Default qRT-PCR



Hello all.
I started my RT-PCR but I was confused with somthing..
I do have 2 house keeping gene, two tissue and 4 genes (8 primers)
I want to do 4 dilution and undiluted sample for each gene
which sample should I assigned as standered and unkown to have a standered curve
and what is the intial concentration for each sample diluted and undiluted as I was asked by the program
I need help please as soon as possible
do you know any helpful website as well

thank and all
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Old 03-20-2011, 01:43 AM
Pipette Filler
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Default Re: qRT-PCR

When I set the samples in the machine it say standard sample should have concentration above zero
and I get a box with the following equation 1.E+06
what is this equation and how i use it to proced with next step
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