| | Re: Replacing Taq with Pfu in PCR reaction
i did use pfu( Fermentas) to amplify a band after i optimized the condition using taq. but the result is not good. multiple bands were obtained although only single band was produced by taq. pfu not works well for my case, i think proble due to the pfu.because i try a few enzyme to amplify the same product(2.5kb). pfu took me 4-5 hours, and the yield very low, not suitable for cloning.
now i m looking for other enzyme with high fidelity and proofreading activity to amplify the product. hope someone can tell me what enzyme is good for my case, thank you.