I'm going to do my RT-PCR. but before that I have to synthesis my cDNA. The problem is that I have a low concentration of RNA. For example, I have a concentration of 44.3ng/ul in 35 ul of sample (S1, so total yield of RNA is 1.5ug and 31.5ng/ul in 35ul of sample (S2) so, total yield of RNA is 1.1ug. Both S1 and S2 are from the same samples. THen, the protocol from the bioline (from cDNA synthesis kit) used 1ug of RNA in a total volume of 10ul of DEPC water. Can someone help me on how to synthesis my cDNA.. Can I mix S1 with S2 so that I can have a more high yield of RNA??