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Problem with site-directed mutagenesis PCR

Problem with site-directed mutagenesis PCR - PCR - Polymerase Chain Reaction Forum

Problem with site-directed mutagenesis PCR - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 01-20-2011, 08:20 AM
Pipette Filler
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Default Problem with site-directed mutagenesis PCR



Hi,
I have a problem with site-directed mutagenesis which after DNA sequencing my sequence appears to have a repeated sequence from my primers but not the whole primers. The position that I have problem with used to be changed with another primers in the same amino acid position (but at that time there's no problem). Have someone faced the same problem as me and how do you guy solve this?

Thanks
Wichai
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