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PCR Primer Dilution

PCR Primer Dilution - PCR - Polymerase Chain Reaction Forum

PCR Primer Dilution - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 12-19-2010, 02:02 PM
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Arrow PCR Primer Dilution



PCR Primer Dilution

How to Dilute Your PCR Primers EASY

If you get your PCR primers lyophilized (ie dry powder), you can re suspend it to create a 100 uM/L concentration.


How to Prepare PCR PRIMER STOCK

For an amount of 38.5 nM of lyophilized primer provided by the primer supplier, one adds 385 microlitres of double distilled water or Tris buffer (some use TRIS EDTA - BUT note that EDTA will inhibit the PCR reaction due to Mg binding - so better to use TRIS buffer) buffer to get 100 uM/L stock solution.


How to Prepare PCR PRIMER WORKING SOLUTION

To prepare 10 microM/L (or 10 picom/ul) as a PCR working solution, one takes 10 microL stock solution and one adds this to 90 microL of Tris buffer or double distilled water to generate a 10X dilution (thus going from 100 to 10 microM).

No calculations needed!

Last edited by admin; 12-19-2010 at 02:20 PM.
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