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| PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory. |
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#2
12-13-2010, 01:11 PM
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 Re: PCR product and sequencing DNA is quite stable, so you can store it a few weeks without compromising DNA. Anyway, don't thaw and refreeze too many times, as freeze/thaw cycles can degrade your PCR product.  |
| The Following User Says Thank You to SebaQ For This Useful Post: | ||
admin (12-14-2010)
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#4
12-14-2010, 12:14 PM
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| Re: PCR product and sequencing Dear Friends, Thanks a lot. Yesterday I received the result of sequencing. The result was perfect. A hug |
| The Following User Says Thank You to senun For This Useful Post: | ||
admin (12-14-2010)
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#7
01-28-2013, 08:41 AM
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| Re: PCR product and sequencing [Only registered users see links. ]You would be amazed at how often a PCR reactions SEEMS to produce the correct band, when in fact it has amplified something spurious. Sometimes when you sequence the band, you will discover that the sequence is completely unexpected and nonsensical. At other times, sequencing with one of your PCR primers will give a completely blank lane, while the other primer will give two simultaneous and superimposed (and thus unreadable) sequences. |
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| pcr , product , sequencing |
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