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PCR product and sequencing

PCR product and sequencing - PCR - Polymerase Chain Reaction Forum

PCR product and sequencing - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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  #1  
Old 11-26-2010, 07:28 PM
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Default PCR product and sequencing



Dears Friends,

How long I can store a PCR product before purify and sequence. Thanks
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Old 12-13-2010, 02:11 PM
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Default Re: PCR product and sequencing

DNA is quite stable, so you can store it a few weeks without compromising DNA.

Anyway, don't thaw and refreeze too many times, as freeze/thaw cycles can degrade your PCR product.
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Old 12-14-2010, 04:42 AM
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Default Re: PCR product and sequencing

I know. Thans a lot!
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Old 12-14-2010, 01:14 PM
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Default Re: PCR product and sequencing

Dear Friends,

Thanks a lot. Yesterday I received the result of sequencing. The result was perfect.

A hug
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Old 12-15-2010, 12:59 PM
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Default Re: PCR product and sequencing

So glad you had a perfect result. Greetings!
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Old 12-16-2010, 07:07 AM
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Default Re: PCR product and sequencing

Glad to hear that!
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Old 01-28-2013, 09:41 AM
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Default Re: PCR product and sequencing

[Only registered users see links. ]You would be amazed at how often a PCR reactions SEEMS to produce the correct band, when in fact it has amplified something spurious. Sometimes when you sequence the band, you will discover that the sequence is completely unexpected and nonsensical. At other times, sequencing with one of your PCR primers will give a completely blank lane, while the other primer will give two simultaneous and superimposed (and thus unreadable) sequences.
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