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senun 11-26-2010 07:28 PM

PCR product and sequencing
 
Dears Friends,

How long I can store a PCR product before purify and sequence. Thanks

SebaQ 12-13-2010 02:11 PM

Re: PCR product and sequencing
 
DNA is quite stable, so you can store it a few weeks without compromising DNA.

Anyway, don't thaw and refreeze too many times, as freeze/thaw cycles can degrade your PCR product.

kaierwen 12-14-2010 04:42 AM

Re: PCR product and sequencing
 
I know. Thans a lot!

senun 12-14-2010 01:14 PM

Re: PCR product and sequencing
 
Dear Friends,

Thanks a lot. Yesterday I received the result of sequencing. The result was perfect.

A hug

SebaQ 12-15-2010 12:59 PM

Re: PCR product and sequencing
 
So glad you had a perfect result. Greetings!

kaierwen 12-16-2010 07:07 AM

Re: PCR product and sequencing
 
Glad to hear that!

davidflora 01-28-2013 09:41 AM

Re: PCR product and sequencing
 
[Only registered and activated users can see links. Click Here To Register...]You would be amazed at how often a PCR reactions SEEMS to produce the correct band, when in fact it has amplified something spurious. Sometimes when you sequence the band, you will discover that the sequence is completely unexpected and nonsensical. At other times, sequencing with one of your PCR primers will give a completely blank lane, while the other primer will give two simultaneous and superimposed (and thus unreadable) sequences.


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