PCR product and sequencing
 

Dears Friends,

How long I can store a PCR product before purify and sequence. Thanks

Re: PCR product and sequencing
 

DNA is quite stable, so you can store it a few weeks without compromising DNA.

Anyway, don't thaw and refreeze too many times, as freeze/thaw cycles can degrade your PCR product.

Re: PCR product and sequencing
 

I know. Thans a lot!

Re: PCR product and sequencing
 

Dear Friends,

Thanks a lot. Yesterday I received the result of sequencing. The result was perfect.

A hug

Re: PCR product and sequencing
 

So glad you had a perfect result. Greetings!

Re: PCR product and sequencing
 

Glad to hear that!

Re: PCR product and sequencing
 

[Only registered and activated users can see links. Click Here To Register...]You would be amazed at how often a PCR reactions SEEMS to produce the correct band, when in fact it has amplified something spurious. Sometimes when you sequence the band, you will discover that the sequence is completely unexpected and nonsensical. At other times, sequencing with one of your PCR primers will give a completely blank lane, while the other primer will give two simultaneous and superimposed (and thus unreadable) sequences.