Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > PCR - Polymerase Chain Reaction Forum
Register Search Today's Posts Mark Forums Read

PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


Primer length question

Primer length question - PCR - Polymerase Chain Reaction Forum

Primer length question - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 10-31-2010, 07:01 AM
Pipette Filler
Points: 188, Level: 3 Points: 188, Level: 3 Points: 188, Level: 3
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2010
Posts: 5
Thanks: 0
Thanked 0 Times in 0 Posts
Default Primer length question



Hi everyone, I'm stuck on this homework question, I'm hoping you could give me some ideas for it.

"In most PCR protocols, the primer pair need only be around 20 nucleotides each in length and annealing temperature is usually 55C; but in site-directed mutagenesis, the primers are often much longer with lengths from 25 to 45 nucleotides and the annealing temperature is around 65C. Two reasons why the primers need to be longer for site-directed mutagenesis?"

The only thing I can think of is that the mutations need to be a fair way in from the ends any other suggestions

Thanks
Reply With Quote
  #2  
Old 11-05-2010, 06:36 PM
Graduate Student
Points: 2,626, Level: 33 Points: 2,626, Level: 33 Points: 2,626, Level: 33
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2010
Location: Santiago, Chile
Posts: 100
Thanks: 1
Thanked 24 Times in 24 Posts
Default Re: Primer length question

Hope i'm on time already... but you shoul read this, the paper of the guys who created this mutation system on E. coli. Inside it's explained everything you need to know:

[Only registered users see links. ]

Hope it helps ^^!

If you still have some questions, feel free to ask or send me a private message
Reply With Quote
The Following User Says Thank You to SebaQ For This Useful Post:
admin (12-14-2010)
  #3  
Old 12-14-2010, 06:47 AM
Pipette Filler
Points: 78, Level: 1 Points: 78, Level: 1 Points: 78, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2010
Posts: 5
Thanks: 0
Thanked 1 Time in 1 Post
Default Re: Primer length question

A very good material! Thanks!
Reply With Quote
Reply

Tags
length , primer , question


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Moving Dimensions Theory Book Due Out in Fall 05--Very Rough Draft: 4th Dimensions Expanding Relative to 3 Spatial Dimensions jollyrogership@yahoo.com Physics Forum 64 03-31-2012 10:24 AM
Simply put, MOVING DIMENSIONS THEORY is THE NEW MODEL: http://physicsmathforums.com drelliot@gmail.com Physics Forum 0 08-29-2006 06:32 AM
Moving Dimensions Theory!! Rock On!! drelliot@gmail.com Physics Forum 1 07-06-2006 05:19 PM
GNU units and units.dat; Units of Measurement and Unit Conversion James Redford Physics Forum 0 07-31-2005 12:08 PM


All times are GMT. The time now is 10:37 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.17763 seconds with 16 queries