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#1
10-14-2010, 10:31 AM
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 Universal primers - HELP! Hi, I am trying to isolate mitochondrial DNA from sardines for my Masters thesis and I would like to use universal primers for cytB from Kochzius, M. et al but I am puzzled. The primers are as follows CytbF (5'-GCC TGA TTC GGA ATA TGC AYG CNA AYG G-3') and CytbR (5'-GGG AAT GGA TCG TAG AAT TGC RTA NGC RAA-3'). I have no idea what the letters N, Y and R stand for, and neither does my mentor, so I would greatly appreciate if somebody could explain this to me as I would like to order them and use them A.S.A.P. Thanks in advance  |
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#2
10-14-2010, 09:11 PM
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| Re: Universal primers - HELP! N - denotes any nucleotide of 4 (A T G C) Y - is either C or T R - stands for A or G When you order your primers is not a problem for the manufacturer to understand this code. |
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| primers , universal , universal primers |
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