I am trying to isolate mitochondrial DNA from sardines for my Masters thesis and I would like to use universal primers for cytB from Kochzius, M. et al
but I am puzzled.
The primers are as follows CytbF (5'-GCC TGA TTC GGA ATA TGC AYG CNA AYG G-3') and CytbR (5'-GGG AAT GGA TCG TAG AAT TGC RTA NGC RAA-3').
I have no idea what the letters N, Y and R stand for, and neither does my mentor, so I would greatly appreciate if somebody could explain this to me as I would like to order them and use them A.S.A.P.
Thanks in advance