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Universal primers - HELP!
I am trying to isolate mitochondrial DNA from sardines for my Masters thesis and I would like to use universal primers for cytB from Kochzius, M. et al but I am puzzled.
The primers are as follows CytbF (5'-GCC TGA TTC GGA ATA TGC AYG CNA AYG G-3') and CytbR (5'-GGG AAT GGA TCG TAG AAT TGC RTA NGC RAA-3').
I have no idea what the letters N, Y and R stand for, and neither does my mentor, so I would greatly appreciate if somebody could explain this to me as I would like to order them and use them A.S.A.P.
Thanks in advance
Re: Universal primers - HELP!
N - denotes any nucleotide of 4 (A T G C)
Y - is either C or T
R - stands for A or G
When you order your primers is not a problem for the manufacturer to understand this code.
|primers , universal , universal primers|
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