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Universal primers - HELP!

Universal primers - HELP! - PCR - Polymerase Chain Reaction Forum

Universal primers - HELP! - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 10-14-2010, 10:31 AM
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Default Universal primers - HELP!



Hi,
I am trying to isolate mitochondrial DNA from sardines for my Masters thesis and I would like to use universal primers for cytB from Kochzius, M. et al but I am puzzled.
The primers are as follows CytbF (5'-GCC TGA TTC GGA ATA TGC AYG CNA AYG G-3') and CytbR (5'-GGG AAT GGA TCG TAG AAT TGC RTA NGC RAA-3').
I have no idea what the letters N, Y and R stand for, and neither does my mentor, so I would greatly appreciate if somebody could explain this to me as I would like to order them and use them A.S.A.P.

Thanks in advance
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Old 10-14-2010, 09:11 PM
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Default Re: Universal primers - HELP!

N - denotes any nucleotide of 4 (A T G C)
Y - is either C or T
R - stands for A or G

When you order your primers is not a problem for the manufacturer to understand this code.
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