| | Re: pcr non specific amplification
There are many reasons for non specific amplification. Try to describe more exactly your pcr conditions (protocoll, molarity of primers, dntp, enzyme, amount of dna, type of dna, age of dna and primer and enzyme, storage conditions (buffer, temperature) of your components, molarity of monovalent and divalent salts you use, cycle number). Have you got any positive control. Is it working. What's with you negative control. Are there bands on gel ? The nonspecific bands youre are speaking of: Are they lower or upper of your desired band ?