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Transfection.. Help!

Transfection.. Help! - PCR - Polymerase Chain Reaction Forum

Transfection.. Help! - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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  #1  
Old 10-06-2010, 02:48 AM
Pipette Filler
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Default Transfection.. Help!



I didn't know where to post this, so, sorry if this does not pertain to anything you're trying to answer.

I'm attempting to travel down the broad road of genetic engineering. Yes, I know, this is not a simple field. I've modified bacteria, and small, uni-cellular organisms. Now, i want to move onto a larger, visible to the eye organism. This may sound rather straight forward, but I would like to cut to the chase. I want to make a plant, almost any species literally glow in the dark. Such labs have already been conducted, such as the glow in the dark rats, using GFP. Using the firefly luciferase gene, is what my research has shown prudent towards being the most easy.
How do I attempt this, is my basic question. I have got lab equipment available
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Old 10-06-2010, 04:40 PM
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Default Re: Transfection.. Help!

Google Tobacco plant expressing GFP.
I knew I'd seen a green flourescing plant somewhere, just haven't read the actual literature about it.

Here are the google images results ---> [Only registered users see links. ]
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Old 10-07-2010, 05:05 PM
Pipette Filler
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Default Re: Transfection.. Help!

The tobacco plant, is actually what inspired this. I somewhat went off on a wim. But i would like to take that brief act of spontaneity and turn it into something great.
I don't even know how to begin this project, which plant to use, and who to contact. ugh!
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Old 10-07-2010, 06:11 PM
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Post Re: Transfection.. Help!

Quote:
Originally Posted by lwpolkadot View Post
The tobacco plant, is actually what inspired this. I somewhat went off on a wim. But i would like to take that brief act of spontaneity and turn it into something great.
I don't even know how to begin this project, which plant to use, and who to contact. ugh!
For know-how either read the methods of the actual articles or contact authors.
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Old 10-08-2010, 05:13 AM
Pipette Filler
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Default Re: Transfection.. Help!

How do you suggest i do that? I don't know who to contact exactly...
Thanks for the replies.
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