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similar Tm for NTC and samples

similar Tm for NTC and samples - PCR - Polymerase Chain Reaction Forum

similar Tm for NTC and samples - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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  #1  
Old 07-19-2010, 10:47 PM
Pipette Filler
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Default similar Tm for NTC and samples



Hi,
I am getting similar Tm values for my negative control and samples; the dissociation peaks are close to each other. Does it mean that I have low gene conc. in my samples? Any help will be much appreciated.
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Old 07-26-2010, 11:48 AM
ahs ahs is offline
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Default Re: similar Tm for NTC and samples

It maybe shows you contamination, or primer dimer. Have a look at your samples in a gel. Compare samples with NTC, find out about the size of products, then you can be sure....
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