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PCR was working but now multiple bands

PCR was working but now multiple bands - PCR - Polymerase Chain Reaction Forum

PCR was working but now multiple bands - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 07-05-2010, 02:30 PM
Pipette Filler
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Default PCR was working but now multiple bands



Hi!

I have the following problem with my PCRs: RT-PCR was set up for the detection of different peptide hormone receptor mRNAs, and was working for weeks without any problem, but suddenly nothing is working exept b-actin. I changed everything (buffer, dNTP, MgCL2, water, new primer dilution, enzyme); I tried another PCR machine (from the same "noname" company) than I get PCR products but with multiple smaller and longer bands together with the right size product. Do you have any suggestions what could be the problem? Thanks in advance!
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