Hey there, I 'm posting a question for a friend of mine here at the lab.
He's running PCR for detection of Trypanosoma cruzi, from blood samples of infected dogs. The thing is he gets a lot of smear, non specific bands I mean. He also sometimes gets the band he' s looking for. But it's really hard to know if it is a consistent result, because of all of the other bands he keeps geting.
He told me that he tried lowering the Mg and it was worst! Also, when he adds a larger sample in the PCR, he gets a more distinct band for T. cruzi but also more non specific bands.
We think that, evidently, all of the bands he's seeing, come from dog's DNA. But how could he prevent this? Or how could he enlarge the T. cruzi DNA in his samples..?
What dou you think he could do?
Thanks so much!