Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > PCR - Polymerase Chain Reaction Forum
Register Search Today's Posts Mark Forums Read

PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


Positive control band is higher than expexcted

Positive control band is higher than expexcted - PCR - Polymerase Chain Reaction Forum

Positive control band is higher than expexcted - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 06-20-2010, 12:44 AM
Pipette Filler
Points: 754, Level: 15 Points: 754, Level: 15 Points: 754, Level: 15
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2009
Location: Shah Alam
Posts: 8
Thanks: 0
Thanked 1 Time in 1 Post
Default Positive control band is higher than expexcted



Hye,

I've got problem with my my positive control. My positive control is 500bp (using template and primers from the kit). But recently, i've always got higher value than that about 600bp sometimes 700bp. I've tried to change the water, buffer, dNTPs, but the result is the same. Previously, didn't have much problem with that. But after about 10th times runnning, the product increased.
Reply With Quote
  #2  
Old 06-22-2010, 04:38 PM
Volunteer
Points: 1,375, Level: 21 Points: 1,375, Level: 21 Points: 1,375, Level: 21
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2009
Posts: 48
Thanks: 10
Thanked 9 Times in 8 Posts
Default Re: Positive control band is higher than expexcted

Is it possible that your lane markers are degraded such that they run faster than expected?
Reply With Quote
Reply

Tags
band , control , expexcted , higher , positive


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
is higher Ct for internal control compared to the target gene is acceptable? deepapmb Real-Time PCR and Quantitative PCR Forum 0 05-22-2010 10:51 AM
negative control gives band same as positive control adiza PCR - Polymerase Chain Reaction Forum 4 04-26-2010 06:03 PM
Positive control for Myc antibody fabianh@umich.edu Protocols and Methods Forum 0 07-05-2007 10:09 PM
dsRNAi positive control Man Mohan Drosophila Forum 0 10-23-2004 04:17 PM
Positive and Negative Control agy lee Microbiology Forum 1 12-18-2003 07:39 PM


All times are GMT. The time now is 08:03 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11781 seconds with 16 queries