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primer dimer goddess!

primer dimer goddess! - PCR - Polymerase Chain Reaction Forum

primer dimer goddess! - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 06-17-2010, 12:24 PM
ira ira is offline
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Default primer dimer goddess!



Can someone please tell me why am i constantly getting primer dimers and no product. I've tried using as little primer as possible but still no PCR product... what could be the problem? could it be my gene? is it too stubborn?
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Old 06-18-2010, 01:17 PM
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Default Re: primer dimer goddess!

What is your template? Although it does matter what concentration/types of different things you use in your reaction, try adding 5% DMSO. It's the god of non-working PCRs (sometimes)!
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Old 07-19-2010, 10:28 PM
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Default Re: primer dimer goddess!

1. Use BSA or t4 gene 32 protein
2. Minimize primer conc.
3. Use touchdown protocol
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