I have a thing going on with my PCR! My negative controls, both the mix one and the one when you add the DNA sample, show contamination. But the thing is the band (around 400 bp) is different that the target sequence. I'm typifing Trypanosoma cruzi from bugs' rectal ampoule samples, so I do a series of PCR with different target sequence but this bands keeps showing up!
I guess my two mail questions are:
1-What is going on?!
2-Is this considered contamination? Because, I mean, the band size is different that the positive bands you get if you have any type of T. cruzi...
Thanks a lot!