Can someone please help me on this
I am working on this pcr
setup where I have three pcr
primers that will amplify 3
exonic regions of the human
genomic DNA given by a customer.
The customer didn't submit any
control dna, so my boss said to
use any control DNA I have
available. I did so, using a
bacterial dna (S. aureus strain) as my control, and
I have product in the control
dna from each of the 3 primers.
what I don't understand is how
the control dna gave pcr product
when it is a different species
from my customer dna and also
because the pcr primers amplify
specific exonic regions, how did
the control dna also show amplified material?