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Multiplex PCR

Multiplex PCR - PCR - Polymerase Chain Reaction Forum

Multiplex PCR - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 04-09-2010, 11:21 AM
Pipette Filler
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Default Multiplex PCR



In general I have DNA and several pairs of primers (R and F). I can't to determine temperature melting because of too many programms which give different results. I used formula Tm= 4*(G+C)+2*(A+T). I'd like to know which difference can be in Tm of primers in the pair (max and min) and in what case I can use several pairs of primers in one PCR. What disadvantages has multiplex PCR? And will it useful if I want to perform PCR gDNA and several pairs of primers?
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