Comparison of nested PCR and Real time PCR

[alomatxon]

Hej Everybody,

Can you help me to answer following questions?

What is similarities, differences, advantages between nested PCR and Real time PCR?

I am looking forward for your soonest response.

Thanks a lot in advance

[butters]

this is long assay .... for me, i try but may make error during this write-up

Nested PCR - 2 pcr steps: 1st normal pcr amplify longer template, 2nd pcr amplify the specific region within 1st pcr amplicon. Usually to amplify something really low copy number.

real time pcr - monitoring of pcr amplification realtime via sybergreen, taqman basically fluorescent dye.

similarity=both utilizing pcr technique, but detection method is slightly different.

I would suggest, u have a look at wiki.... it will be faster than me and anybody here doing summary... Once u got specific aspect like which method is best to use for detection of so and so we could help more.

*sweat also fainted coz my internet connection got problem... lucky i don't have to rewrite this again~