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Comparison of nested PCR and Real time PCR
Can you help me to answer following questions?
What is similarities, differences, advantages between nested PCR and Real time PCR?
I am looking forward for your soonest response.
Thanks a lot in advance
|The Following User Says Thank You to alomatxon For This Useful Post:|
dr.tahir fareed turk (12-26-2011)
Re: Comparison of nested PCR and Real time PCR
this is long assay .... for me, i try but may make error during this write-up
Nested PCR - 2 pcr steps: 1st normal pcr amplify longer template, 2nd pcr amplify the specific region within 1st pcr amplicon. Usually to amplify something really low copy number.
real time pcr - monitoring of pcr amplification realtime via sybergreen, taqman basically fluorescent dye.
similarity=both utilizing pcr technique, but detection method is slightly different.
I would suggest, u have a look at wiki.... it will be faster than me and anybody here doing summary... Once u got specific aspect like which method is best to use for detection of so and so we could help more.
*sweat also fainted coz my internet connection got problem... lucky i don't have to rewrite this again~
Last edited by butters; 03-14-2010 at 03:25 PM.
|comparison , nested , pcr , real , time|