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Heavy and Light DNA strand calculation... help!

Heavy and Light DNA strand calculation... help! - PCR - Polymerase Chain Reaction Forum

Heavy and Light DNA strand calculation... help! - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 01-16-2010, 08:26 AM
Pipette Filler
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Default Heavy and Light DNA strand calculation... help!



Here is the question...
After many generations of growth in medium containing 14N, the medium was replaced with new medium containing 15N.
After 5 generations, what is the ratio of light:heavy DNA strands...?

I answered...
Initial ratio (Light : Heavy) 0:2
End Round 1 2:2
End Round 2 2:6
End Round 3 2:14
End Round 4 2:30
End Round 5 2:54 or 1:27

But this is wrong and I don't know why.....!!!!
Please help.
Thanks.
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calculation , dna , heavy , light , strand


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