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How many DNA molecules would there be after four rounds of PCR...?

How many DNA molecules would there be after four rounds of PCR...? - PCR - Polymerase Chain Reaction Forum

How many DNA molecules would there be after four rounds of PCR...? - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 12-14-2009, 01:18 AM
Pipette Filler
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Default How many DNA molecules would there be after four rounds of PCR...?



if the initial reaction mixture contained two molecules?

a. 8
b. 16
c. 32
d. 64
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Old 01-05-2010, 12:48 AM
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Default Re: How many DNA molecules would there be after four rounds of PCR...?

Hi Stan,

Under 100% efficient conditions, PCR doubles the number of molecules at every cycle. So doing your own math, you should be able to figure out the answer.
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Old 01-12-2010, 04:00 PM
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Default Re: How many DNA molecules would there be after four rounds of PCR...?

for me i would say 2^x, whereas x is number of cycles. Thus when x=4, 2^4=2 x 2 x 2 x 2 = 16.

that is when starting material is 1. however since urs is 2 thus its another additional x2. and the answer is 16x2=32. correct me if i am wrong. C

I just found that a better answer was answer in this post
[Only registered users see links. ]

Last edited by butters; 01-12-2010 at 04:59 PM.
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