Hello everyone, I am just starting learning to design primer. But I still have problem with reverse primer design.
If I have sequence like this
If I use start codon ATG and ACTAGT as restriction enzyme, my forward primer should be 5’ ACTAGT ATG
GAT AGT GGT TGC GTT GTG 3’
If I use TAA as stop codon and CGGCCG as restriction enzyme, my reverse primer should be 5’ GCCGGC AAT
AGC TGT GAC CAA GGA GTT 3’
Please help and I will be happy to have your suggestion. Thank you.