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single cell multiplex qPCR

single cell multiplex qPCR - PCR - Polymerase Chain Reaction Forum

single cell multiplex qPCR - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 12-02-2009, 11:11 PM
Pipette Filler
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Default single cell multiplex qPCR



Hi all,

I am perfoming a single cell multiplex PCR and it is working. I have done several time points analysis. However, I have read that people working on single cells run a standard with mRNAs. I didn't do it because I wondered to do a relative quantification. Is this right or absolute quantification with mRNA standards is a requirement for single cell analysis?

Thanks.
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