How can RT-PCR(reverse transcriptase polymerase chain reaction)be used to asses the

Dreamer_24 · #1 11-13-2009, 02:18 AM

splicing of specific mRNA? I know the very basics of how RT-PCR works so I am not really sure how this would relate to alternatvie splicing. Any information would be helpful.

ScienceChic · #2 11-13-2009, 02:18 AM

mRNA is the result of alternative splicing. Using reverse transcriptase PCR, you would be able to see the sizes of the sequences that were a result of splicing.

Say DNA is transcribed and once in the mRNA form, two splice sites are possible

DNA ========== (this long)
one splice site is after the first segment, so the mRNA would only be =

another splice site is in the middle so you get a sequence that results in about =====

lavendergirl · #3 12-15-2009, 03:17 PM

You could also cut the various mRNA lengths out of the gel and sequence them too. You could also purify and re-amplify for concentrated mRNA.

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How can RT-PCR(reverse transcriptase polymerase chain reaction)be used to asses the

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