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How can RT-PCR(reverse transcriptase polymerase chain reaction)be used to asses the
splicing of specific mRNA? I know the very basics of how RT-PCR works so I am not really sure how this would relate to alternatvie splicing. Any information would be helpful.
mRNA is the result of alternative splicing. Using reverse transcriptase PCR, you would be able to see the sizes of the sequences that were a result of splicing.
Say DNA is transcribed and once in the mRNA form, two splice sites are possible
DNA ========== (this long)
one splice site is after the first segment, so the mRNA would only be =
another splice site is in the middle so you get a sequence that results in about =====
Re: How can RT-PCR(reverse transcriptase polymerase chain reaction)be used to asses t
You could also cut the various mRNA lengths out of the gel and sequence them too. You could also purify and re-amplify for concentrated mRNA.
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